Bacillus Subtilis

Bacillus subtilis n9-1-35 strain was bred by aerospace breeding. Its ability to produce neutral protease increased from 45 international units of the initial strain to 1500 international units, the enzyme production ability increased by 30 ~ 40 times, and the rapid enzyme production period was advanced by 4 hours. It has strong protease and amylase activities, promotes the decomposition of protein into polypeptides, small peptides and amino acids, and decomposes polysaccharides such as starch into glucose, which can make up for the deficiency of animal endogenous enzymes, improve animal digestion ability of feed, especially protein, and improve feed conversion rate by 5 ~ 10%. These are incomparable to the low-cost products of small workshops!

The reason why Bacillus plays such a powerful role depends on spores!You can't just look at the number of bacteria!Because the bacteria content is high, it does not mean that the spore content is high!Bacteria without spores die quickly and have poor efficacy!First, liquid fermentation technology is better than solid fermentation;Second, different process treatments under the same technology affect the spore rate. If the process control is not good, the spore rate is not guaranteed!Third, spore free bacteria die quickly, so the preservation time is short! The Bacillus like Boris was treated by spray drying at high temperature for 4 months, and the survival rate was 1. The survival rate was 100% at room temperature and 12 months at room temperature. The survival rate was still around 90%.Good products always stand the test of time and have a low loss rate!Therefore, it is recommended that you check the number of viable bacteria and spore content at regular intervals to compare the advantages and disadvantages.

Some manufacturers will take the initiative to let consumers use the water-soluble method to detect the spore content to show that their strains are good, but this method is unscientific. Because many spores contain bacteriophages, generally speaking, they eat spores. They are attached to the spore, and once the spore is eaten by it, the strain will quickly inactivate! Therefore, when testing, we should also refer to the national standard gb20287-2006 agricultural microbial agent testing, measurement and comparison. If the results are very different, it is a problematic product. For example, the number of bacteria detected is 50 billion / g, but the number of spores is more than 55 billion / g, indicating that there are many bacteriophages in this strain! Under normal conditions, the number of spores < the number of bacteria, but the difference value should not be too large. If 20 billion spores and 30 billion bacteria fail to meet the standard, the bacteria will die quickly!

It is not only the performance of the strain itself, but also the rich metabolites produced by it, such as enzymes, organic acids, vitamins, physiological activities and other substances, which play an important role. Therefore, the content, activity and quality of fermentation metabolites are also important criteria to prove whether bacillus is good or not!The growth of metabolites is not only inseparable from the necessary conditions such as nutrition, temperature, humidity and pH, but also positively related to the equipment, process, technology and other factors of synthetic reproduction! If the technological treatment is not in place, it will seriously destroy various metabolic active components produced in the fermentation process!

In general, if you want to buy high-quality bacillus, you must choose products with high number of bacteria + high spore rate + low miscellaneous bacteria rate + rich metabolites + stable quality + good brand reputation!

In addition, when buying a good bacillus, the dosage, stage, storage and other factors used by consumers will also affect the final use effect. Therefore, it is recommended to use and store it according to the product instructions of large brands of regular manufacturers or under the guidance of professional technicians.