In the present protocol, the primers for the 1st PCR must be designed to amplify approximately 1 kb upstream and downstream flanking regions of the target region in the genome. Such long flanking sequences are necessary to improve the efficiency of homologous recombination.
Read MoreAvian influenza is a kind of poultry infection syndrome caused by type A influenza virus, which seriously threatens the global breeding industry, human health and public health safety.
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